1. High‑Resolution Scientific‑Grade Cooled Camera
Equipped with an approximately 6‑megapixel, 16‑bit scientific‑grade cooled camera, it delivers abundant spatial details while maintaining high sensitivity. Deep cooling reduces background noise generated during long‑exposure imaging, satisfying imaging requirements for both BLI and FLI modes.
2. Bioluminescence Imaging Module
A fully light‑tight environment combined with a high‑transmission lens detects faint photon signals produced by luciferase reactions. Adjustable exposure time, pixel binning and lens aperture settings are supported to adapt to diverse experimental conditions ranging from strong signals to low‑expression models.
3. Multi‑Band Fluorescence Excitation Module
It is fitted with 20 monochromatic LED light sources across 24 excitation positions, together with up to 18 narrow‑band emission filters of distinct wavelengths covering visible light to near‑infrared‑I (NIR‑I) range, enabling detection of fluorescence signals from various fluorescent proteins and fluorescent probe labels.
Each channel can be independently controlled via software and is compatible with GFP, RFP, Cy5, Cy7, ICG and other widely‑used fluorescent labels.
4. Motorized Emission Filter System
The 24‑position motorized filter wheel automatically switches emission channels according to experimental protocols. Narrow‑band filters minimize excitation‑light leakage and crosstalk among different fluorescence signals, laying the foundation for multi‑channel imaging and spectral analysis.
5. Spectral Unmixing and Background Subtraction
Based on spectral signatures of different fluorescent components, the software separates target signals, tissue autofluorescence and multi‑label signals, improving image contrast in scenarios with strong tissue autofluorescence or overlapping probe spectra.
6. Digital X‑ray Imaging Module
A micro‑focus X‑ray source and flat‑panel detector are configured to clearly visualize skeletal anatomy, body contours and high‑density implanted materials of laboratory animals. Tube voltage and tube current can be adjusted according to animal species and imaging regions. Automatic fusion between optical and X‑ray images is available.
BLI, FLI and X‑ray share one single animal stage; no animal transfer is required during acquisition. The software performs automatic image registration using fixed spatial coordinates to eliminate errors from manual overlay.
7. Motorized Stage and High‑Throughput Imaging
The motorized stage supports multiple field‑of‑view presets, autofocus and animal position adjustment. It enables local imaging for a single animal as well as batch imaging of up to 5 mice or 3 rats.
8. Animal Anesthesia and Temperature Maintenance
Integrated temperature‑controlled animal platform, multi‑channel anesthesia nose cones and waste‑gas recovery interface keep animals at relatively stable body temperature, posture and anesthesia status across multiple imaging time points.
9. Integrated Analysis Software
The software supports optical/X‑ray image fusion, transparency adjustment, pseudo‑color display, ROI quantification and multi‑time‑point comparison.
10. X‑ray Safety Protection
The system is equipped with a shielding enclosure, door safety interlock, operating status indicators and emergency‑stop function. The X‑ray module can only be activated when the chamber door is fully closed and all safety conditions are met.
